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retinal pigment epithelium cell line  (ATCC)


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    Structured Review

    ATCC retinal pigment epithelium cell line
    Retinal Pigment Epithelium Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 2233 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/htert+rpe+1/hTERT+RPE-1/pm42316211-53-2-9
    Average 99 stars, based on 2233 article reviews
    retinal pigment epithelium cell line - by Bioz Stars, 2026-09
    99/100 stars

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    Cell Culture:

    Article Title: Topological stress regulates replication fork dynamics in unperturbed S phase.
    Article Snippet: .. Cell culture AR TI CL E IN P RE SS U2OS (osteosarcoma; human, female origin, ATCC, HTB-96), HCT116 (colorectal carcinoma; human, male origin, ATCC, CCL-247), hTERT-RPE-1 (immortalized retinal pigment epithelium; human, female origin, ATCC, CRL-4000; referred to as RPE-1 throughout the manuscript), U2OS cells stably overexpressing N-terminal GFP-tagged PRIMPOL80 (referred to as U2OSPPOL), RPE1_Cyclin E_P53KO cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM; Invitrogen, 11960) supplemented with 10% fetal bovine serum (FBS; 10500) and 1% penicillin-streptomycin (Invitrogen, 10378016) under standard conditions (37 °C, 5% CO2, humidified atmosphere). ..

    Article Title: Selective autophagy of whole micronuclei suppresses chromosomal instability
    Article Snippet: .. hTERT RPE-1, U2OS, HeLa and HEK293T cells were purchased from the American Type Culture Collection (ATCC) and cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) GlutaMAX (Gibco) supplemented with 10 % fetal bovine serum (FBS) (Gibco) and 1% penicillin/streptomycin. ..

    Article Title: Cell Cycle‐Specific Regulation of Centrosome Clustering Dynamics in Cancer Cells by the Multifunctional Kinesin HSET
    Article Snippet: MDA‐MB‐231 (BCRC, 60,425) cells were cultured in RPMI (Gibco, 11875085) supplemented with 1 mM sodium pyruvate, and 10% fetal bovine serum (Merck). .. Hep3B (ATCC, HB‐8064) cells were grown in MEM (Gibco, 11095072) supplemented with 1 m m sodium pyruvate and 10% fetal bovine serum (Merck). hTERT‐RPE‐1 (ATCC, CRL‐4000) cells were cultured in DMEM/F12 (Gibco, 11320033) supplemented with 10% fetal bovine serum (Merck). .. Genes encoding the proteins of interest were amplified by PCR using Phusion polymerase (NEB) and cloned into a mammalian expression vector under the control of a CMV promoter via the In‐Fusion cloning method (Takara).

    Article Title: Microfluidic analysis reveals ROCK2 regulation of endothelial cilia is essential for blood vessel lumen formation and vascular integrity
    Article Snippet: .. The following cell lines were cultured in a humidified atmosphere at 37°C with 5% CO 2 . hCMEC/D3 (human cerebral microvascular endothelial cells) (ATCC® CRL-3245TM) were cultured in EndoGRO-MV Complete Media (Merck, SCME004) supplemented with 1 ng/ml basic Fibroblast Growth Factor (bFGF; PeproTech, 100-18B) and used between passages 30-35 for ciliogenesis assays. bEnd.3 (mouse brain endothelial cells) (ATCC® CRL-2299TM) were cultured in DMEM (Gibco, 11995065) supplemented with 10% (v/v) heat-inactivated foetal bovine serum (FBS) (Merck, F9665), 100 U/ml penicillin, 100 μg/ml streptomycin, and 2 mM L-glutamine (all from Gibco). hTERT RPE-1 (human retinal pigment epithelial cells) (ATCC® CRL-4000TM) were cultured in DMEM/F12 (Gibco, 11330032) supplemented with 10% FBS, penicillin/streptomycin, and L-glutamine. ..

    Article Title: LBR and LAP2 mediate heterochromatin tethering to the nuclear periphery to preserve genome homeostasis.
    Article Snippet: The secondary antibodies used included mouse Alexa Fluor 488 (ThermoFisher Scientific, A-11001), mouse Alexa Fluor 594 (ThermoFisher Scientific, A-11005), rabbit Alexa Fluor 488 (ThermoFisher Scientific, A-11034), rabbit Alexa Fluor 594 (ThermoFisher Scientific, A-11012), human Alexa Fluor 647 (ThermoFisher Scientific, A-21445) used for immunofluorescence at 1:300, and mouse Alexa Fluor Plus 680 (ThermoFisher Scientific, A32729) and rabbit Alexa Fluor Plus 800 (ThermoFisher Scientific, A32735) used for blotting at 1:10,000. .. Cell lines and cell culture HCT116 (CCL-247; ATCC), hTERT-RPE-1 (kind gift from J. Mansfeld, Institute of Cancer Research, London) and NIH/3T3 (kind gift from V. Vogel, ETHZ) cells were grown in Dulbecco’s modified Eagle medium (DMEM; Gibco), supplemented with 10% fetal calf serum (FCS; Eurobio Scientific) and 100 μg ml−1 penicillin/streptomycin (Corning) at 37 °C, 5% CO2. .. ES-E14TG2a mESCs (CRL-1821; ATCC) were grown in DMEM (Sigma) supplemented with 13% FCS (Sigma), 0.32% 2-mercaptoethanol (Gibco) and 100 μg ml−1 penicillin/streptomycin (Corning).

    Stable Transfection:

    Article Title: Topological stress regulates replication fork dynamics in unperturbed S phase.
    Article Snippet: .. Cell culture AR TI CL E IN P RE SS U2OS (osteosarcoma; human, female origin, ATCC, HTB-96), HCT116 (colorectal carcinoma; human, male origin, ATCC, CCL-247), hTERT-RPE-1 (immortalized retinal pigment epithelium; human, female origin, ATCC, CRL-4000; referred to as RPE-1 throughout the manuscript), U2OS cells stably overexpressing N-terminal GFP-tagged PRIMPOL80 (referred to as U2OSPPOL), RPE1_Cyclin E_P53KO cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM; Invitrogen, 11960) supplemented with 10% fetal bovine serum (FBS; 10500) and 1% penicillin-streptomycin (Invitrogen, 10378016) under standard conditions (37 °C, 5% CO2, humidified atmosphere). ..

    Modification:

    Article Title: Topological stress regulates replication fork dynamics in unperturbed S phase.
    Article Snippet: .. Cell culture AR TI CL E IN P RE SS U2OS (osteosarcoma; human, female origin, ATCC, HTB-96), HCT116 (colorectal carcinoma; human, male origin, ATCC, CCL-247), hTERT-RPE-1 (immortalized retinal pigment epithelium; human, female origin, ATCC, CRL-4000; referred to as RPE-1 throughout the manuscript), U2OS cells stably overexpressing N-terminal GFP-tagged PRIMPOL80 (referred to as U2OSPPOL), RPE1_Cyclin E_P53KO cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM; Invitrogen, 11960) supplemented with 10% fetal bovine serum (FBS; 10500) and 1% penicillin-streptomycin (Invitrogen, 10378016) under standard conditions (37 °C, 5% CO2, humidified atmosphere). ..

    Article Title: Selective autophagy of whole micronuclei suppresses chromosomal instability
    Article Snippet: .. hTERT RPE-1, U2OS, HeLa and HEK293T cells were purchased from the American Type Culture Collection (ATCC) and cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) GlutaMAX (Gibco) supplemented with 10 % fetal bovine serum (FBS) (Gibco) and 1% penicillin/streptomycin. ..

    Article Title: LBR and LAP2 mediate heterochromatin tethering to the nuclear periphery to preserve genome homeostasis.
    Article Snippet: The secondary antibodies used included mouse Alexa Fluor 488 (ThermoFisher Scientific, A-11001), mouse Alexa Fluor 594 (ThermoFisher Scientific, A-11005), rabbit Alexa Fluor 488 (ThermoFisher Scientific, A-11034), rabbit Alexa Fluor 594 (ThermoFisher Scientific, A-11012), human Alexa Fluor 647 (ThermoFisher Scientific, A-21445) used for immunofluorescence at 1:300, and mouse Alexa Fluor Plus 680 (ThermoFisher Scientific, A32729) and rabbit Alexa Fluor Plus 800 (ThermoFisher Scientific, A32735) used for blotting at 1:10,000. .. Cell lines and cell culture HCT116 (CCL-247; ATCC), hTERT-RPE-1 (kind gift from J. Mansfeld, Institute of Cancer Research, London) and NIH/3T3 (kind gift from V. Vogel, ETHZ) cells were grown in Dulbecco’s modified Eagle medium (DMEM; Gibco), supplemented with 10% fetal calf serum (FCS; Eurobio Scientific) and 100 μg ml−1 penicillin/streptomycin (Corning) at 37 °C, 5% CO2. .. ES-E14TG2a mESCs (CRL-1821; ATCC) were grown in DMEM (Sigma) supplemented with 13% FCS (Sigma), 0.32% 2-mercaptoethanol (Gibco) and 100 μg ml−1 penicillin/streptomycin (Corning).

    Article Title: Proteomic Profiling Reveals How Physiological Media Reshape Cancer Cell Proteomes and Signaling Networks.
    Article Snippet: .. DU145 (human prostate carcinoma, male, ATCC HTB-81, RRID: CVCL_0105), HCT116 (human colorectal carcinoma, male, ATCC CCL-247, RRID: CVCL_0291), HEK293T (human embryonic kidney, fetus, ATCC CCL-3216, RRID: CVCL_0063), HeLa (human uterine adenocarcinoma, female, ATCC CRM-CCL-2, RRID: CVCL_0030), MCF7 (human breast adenocarcinoma, female, ATCC HTB-22, RRID: CVCL_0031), PANC-1(human pancreatic adenocarcinoma, male, ATCC CRL-1469), PC-3 (human prostate adenocarcinoma, male, ATCC CRL-1435, RRID: CVCL_0035), hTERT RPE-1 (human retinal pigment epithelium, ATCC CRL-4000, RRID: CVCL_4388), and U2OS (human osteosarcoma, female, ATCC HTB-96, RRID: CVCL_0042) cells were grown in either Dulbecco’s modified Eagle’s medium (DMEM, high glucose and pyruvate (Gibco)) supplemented with 10% dialyzed fetal bovine serum (Gibco, US origin) or HPLM media (Gibco) and 10% dialyzed fetal bovine serum (Gibco, US origin) for ~15 days (over three passages) prior to the proteomic analyses. ..

    Genetically Modified:

    Article Title: Monoclonal cell lines expressing an exogenous substance and uses thereof
    Article Snippet: .. In an embodiment, the genetically modified cell is derived from a human cell line available from the American Type Culture Collection (Manassas, VA), e.g., the ARPE-19 (ATCC® CRL-2302TM) cell line or the hTERT RPE-1 (ATCC® CRL-4000TM) cell line. ..

    Derivative Assay:

    Article Title: Monoclonal cell lines expressing an exogenous substance and uses thereof
    Article Snippet: .. In an embodiment, the genetically modified cell is derived from a human cell line available from the American Type Culture Collection (Manassas, VA), e.g., the ARPE-19 (ATCC® CRL-2302TM) cell line or the hTERT RPE-1 (ATCC® CRL-4000TM) cell line. ..



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